TABLE 9.

Ideal ratios of amount of proteins to number of protoplasts, and volume of proteins to volume of protoplasts for GFP control and for CRISPR RNPs.

For GFP control	μg of GFP plasmid/total protoplast number	0.0002 to 0.0004
	μl of GFP plasmid/total μl protoplasts	0.4 to 0.6
For RNPs	μg of RNPs/total protoplast number	0.0004 to 0.002
	μl of RNPs/total μl protoplasts	0.2 to 0.4