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. 2020 Jun 10;8:575. doi: 10.3389/fbioe.2020.00575

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Copyright © 2020 Sanches, Geaquinto, Cruz, Schuck, Lorencini, Granjeiro and Ribeiro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Toxicological assays. (A) Physico-chemical characteristics of NPs TiO₂ NPs that possible interfere with the biological assays; that include (B) optical capabilities of TiO₂ NPs such as intrinsic absorbance and/or fluorescence (b1); adsorption of proteins, salts and dyes (s) to NPs (b2); and dissolution of NPs with the consequent release of metal ions to supernatant (b3). (C) Conventional MTT test analyzing TiO₂ NPs demonstrating that NPs can adsorb to MTT dye that avoid metabolization of reagents. Image adapted from: https://smart.servier.com.