Table 2.
Accuracy of mixture estimates by the AQ pyrosequencing assay across different influenza virus types/subtype and PA/I38 substitutions
| Expected variant (%) | Percentage of variant detected by pyrosequencing from a mixed population of WT and indicated PA/I38X variant | |||
|---|---|---|---|---|
| PA/I38T | PA/I38M | PA/I38F | ||
| A(H1N1pdm09) | 0 | 2.4 ± 0.3 | 0 ± 0 | ND |
| A/Perth/261/2009 | 25 | 23.8 ± 0.4 | 23.3 ± 0.3 | ND |
| 50 | 48.8 ± 0.5 | 47.8 ± 0.4 | ND | |
| 75 | 72.7 ± 0.6 | 72.1 ± 0.3 | ND | |
| 100 | 98.3 ± 0.2 | 100 ± 0 | ND | |
| A(H3N2) | 0 | 0.5 ± 0.7 | 1.3 ± 0.1 | ND |
| A/Perth/16/2009 | 25 | 27.3 ± 0.5 | 27.2 ± 0.5 | ND |
| 50 | 52.6 ± 0.3 | 51.9 ± 0.6 | ND | |
| 75 | 73.9 ± 0.4 | 74.3 ± 0.1 | ND | |
| 100 | 98.3 ± 0.4 | 99.3 ± 0.5 | ND | |
| B/Yamagata lineage | 0 | 1.2 ± 1.7 | 0.4 ± 0.6 | 0 ± 0 |
| B/Yamanashi/166/1998 | 25 | 31.7 ± 0.4 | 25.8 ± 0.5 | 9.5 ± 1.3 |
| 50 | 49.7 ± 0.4 | 47.5 ± 0.5 | 35.8 ± 5.5 | |
| 75 | 71.6 ± 0.2 | 72.1 ± 0.5 | 63.7 ± 3.2 | |
| 100 | 98.4 ± 1.7 | 100 ± 0 | 100 ± 0 | |
Not determined (ND) ‐ AQ analysis could not be performed by PyroMarkID software.
Variant percentages are the average ± standard deviation of three experiments.