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. 2020 Apr 29;39(12):e104133. doi: 10.15252/embj.2019104133

Figure EV1. BGL3 directly binds BARD1 and PARP1 in living cells.

Figure EV1

  1. Schematic overview of RAP‐MS.
  2. RT–PCR products of RNA antisense captured lncRNAs were analyzed on an agarose gel. Ladder, 1 kb (Maker1) and 100 bp (Maker2) DNA ladder. F1: BGL3 DNA fragment 1–970 bp, F2: BGL3 DNA fragment 970–2,016 bp, F3: BGL3 DNA fragment 1,905–2,650 bp, F4: BGL3 DNA fragment 3,181–3,696 bp.
  3. LncRNA being pulled down in the “BGL3 antisense group” (BGL3 group) and the control group (RMRP group) was examined by qRT–PCR using three different primers. P1: primer1, P2: primer2, P3: primer3. Data were presented as mean ± SD of three biological replicates and analyzed by two‐tailed Student's t‐test, **P < 0.01.
  4. Overlap of non‐redundant quantified proteins captured by BGL3 in three biological replicates.
  5. Pairwise correlations of protein intensity between replicate experiments. The Pearson correlation coefficient is indicated.
  6. BGL3‐interacting proteins were grouped based on cellular component and biological process pathways by GO analysis.

Source data are available online for this figure.