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Validation of HSV-1 Targeting SpCas9 and SaCas9 gRNAs
(A) Coomassie staining of purified SpCas9 and SaCas9. (B) In vitro cleavage of UL8, UL52, and ICP4 targeted sites by SpCas9 with indicated gRNA. (C) In vitro cleavage of ICP4 targeted sites by SaCas9 with indicated gRNA. (D) T7E1 assay showing cleavage of HSV-1 genome in SpCas9- or SaCas9-expressing cells. Generation of indels are indicated by an asterisk. Ctrl, control.
