Table 2.
List of Antibodies Used for Characterization of Chondrocytes and Chondroprogenitors by Flow Cytometric Analysis.
| Groups | Surface Markers | Fluorochrome Conjugate | Source | |
|---|---|---|---|---|
| Group I: Classical MSC markers | CD105 | Endoglin glycoprotein | FITC | BD Bioscience |
| CD73 | Ecto-5′- nucleotidase | PE | BD Bioscience | |
| CD90 | Thymus cell antigen-1 | PE | BD Bioscience | |
| Group II: Negative MSC markers | CD34 | Hematopoietic stem cell markers | PE | BD Bioscience |
| CD45 | FITC | BD Bioscience | ||
| Group III: Stem cell and chondrocyte markers | CD106 | Vascular cell adhesion molecule-1 (VCAM-1) | PE | Miltenyi Biotec |
| CD54 | Intracellular adhesion molecule-1 (ICAM-1); hylaluronan receptor | PE | Miltenyi Biotec | |
| CD44 | PE | Miltenyi Biotec | ||
| Group IV: Chondroprogenitor-specific markers | CD29 | Integrin beta-1 (Iβ1) | PE | Miltenyi Biotec |
| CD151 | Tetraspanin family | PE | Miltenyi Biotec | |
| CD166 | Activated leucocyte adhesion molecule | PE | Miltenyi Biotec | |
| CD49e | Integrin alpha-5 (Iα5); fibronectin receptor | PE | Miltenyi Biotec | |
| CD146 | Melanoma cell adhesion molecule | PE | Miltenyi Biotec | |
MSC = mesenchymal stem cell; CD = cluster of differentiation; FITC = fluorescein isothiocyanate; PE = phycoerythrin.