Skip to main content
. 2018 Aug 23;11(3):374–384. doi: 10.1177/1947603518796140

Table 4.

Sequence of the Primers Used for RT-PCR.

Gene of Interest Primers (5′-3′) Product Size (bp)
Forward Primer Reverse Primer
MaxiK CGGTTAGTGGAAGAAAGCACA GAGGACGGAACCCTGATAAAA 42
HKv1.1 CCATCATTCCTTATTTCATCAC CTCTTCCCCCTCAGTTTCTC 42
hKv1.4 ACGAGGGCTTTGTGAGAGA TAAGATGACCAGGACGGACA 39
hKv4.2 GCCTTCTTCTGCTTGGACAC TCATCACCAGCCCAATGTAA 40
hEAG1 TGGTCCTGCTGGTGTGTG ACAACGAGGAGATGTAGACAG 39
hEAG2 AGGTCCTACAGTGTTTGTGTATC GAGCTGGAATATGGCGAGAA 43
hNE-Na GCTCCGAGTCTTCAAGTTGG GGTTGTTTGCATCAGGGTCT 40
SCN5A CCTAATCATCTTCCGCATCC TGTTCATCTCTCTGTCCTCATC 42
CACNA1C AAGGCTACCTGGATTGGATCAC GCCACGTTTTCGGTGTTGAC 42
CACNA1G CTTTGCCGAAGGTAGCGCCGAAT GGCACATCTGGTGGGCTCTA 43
GAPDH TCAGCAATGCCTCCTGCAC TCTGGGTGGCAGTGATGGC 117

RT-PCR = reverse transcription-polymerase chain reaction; Maxi K = human large-conductance calcium activated K+ channel; HKv1.1 = voltage gated K+ channel (subfamily A member 1); hKv1.4 = human voltage gated K+ channel (subfamily A member 4); hKv4.2 = human voltage gated K+ channel (subfamily D member 2); hEAG1 = K+ voltage gated channel (subfamily H member 1); hEAG2 = K+ voltage gated channel (subfamily H member 2); hNE-Na = human neuroendocrine tetrodotoxin-sensitive voltage-activated Na+ channel; SCN5A = human cardiac tetrodotoxin-insensitive voltage-dependent Na+ channel; CACNA1C = human voltage-dependent L-type Ca2+ channel; CACNA1G = human voltage-dependent T-type; GAPDH = glyceraldehyde 3-phosphate dehydrogenase.