Table 4.
Gene of Interest | Primers (5′-3′) | Product Size (bp) | |
---|---|---|---|
Forward Primer | Reverse Primer | ||
MaxiK | CGGTTAGTGGAAGAAAGCACA | GAGGACGGAACCCTGATAAAA | 42 |
HKv1.1 | CCATCATTCCTTATTTCATCAC | CTCTTCCCCCTCAGTTTCTC | 42 |
hKv1.4 | ACGAGGGCTTTGTGAGAGA | TAAGATGACCAGGACGGACA | 39 |
hKv4.2 | GCCTTCTTCTGCTTGGACAC | TCATCACCAGCCCAATGTAA | 40 |
hEAG1 | TGGTCCTGCTGGTGTGTG | ACAACGAGGAGATGTAGACAG | 39 |
hEAG2 | AGGTCCTACAGTGTTTGTGTATC | GAGCTGGAATATGGCGAGAA | 43 |
hNE-Na | GCTCCGAGTCTTCAAGTTGG | GGTTGTTTGCATCAGGGTCT | 40 |
SCN5A | CCTAATCATCTTCCGCATCC | TGTTCATCTCTCTGTCCTCATC | 42 |
CACNA1C | AAGGCTACCTGGATTGGATCAC | GCCACGTTTTCGGTGTTGAC | 42 |
CACNA1G | CTTTGCCGAAGGTAGCGCCGAAT | GGCACATCTGGTGGGCTCTA | 43 |
GAPDH | TCAGCAATGCCTCCTGCAC | TCTGGGTGGCAGTGATGGC | 117 |
RT-PCR = reverse transcription-polymerase chain reaction; Maxi K = human large-conductance calcium activated K+ channel; HKv1.1 = voltage gated K+ channel (subfamily A member 1); hKv1.4 = human voltage gated K+ channel (subfamily A member 4); hKv4.2 = human voltage gated K+ channel (subfamily D member 2); hEAG1 = K+ voltage gated channel (subfamily H member 1); hEAG2 = K+ voltage gated channel (subfamily H member 2); hNE-Na = human neuroendocrine tetrodotoxin-sensitive voltage-activated Na+ channel; SCN5A = human cardiac tetrodotoxin-insensitive voltage-dependent Na+ channel; CACNA1C = human voltage-dependent L-type Ca2+ channel; CACNA1G = human voltage-dependent T-type; GAPDH = glyceraldehyde 3-phosphate dehydrogenase.