Fig. 6.

Effect of crosslinking treatments on human dermal fibroblast morphology and spreading. Fibroblast morphology is imaged on non-crosslinked and EDC-NHS, genipin- and TG2-treated collagen films. HDFs are observed to cluster and demonstrate a rounded morphology with increasing EDC-NHS crosslinking, expressing a spindle-like morphology of the filopodia (labelled in Fig. 7a) on the 50% and 100% EDC-NHS treated films. HDFs present in all other conditions are well-spread exhibiting broad lamellipodia. Fibroblasts on genipin and TG2-treated films also possess prominent ventral stress fibres (labelled in Fig. 7b). Genipin-treated films autofluorescence of collagen fibres in the red channel. Red: Phalloidin (actin), blue: DAPI (nucleus). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)