Fig. 3. Delayed administration of 3-MA reduces ECM protein deposition and inhibits hyperuricemia-related renal fibrosis.

Photomicrographs illustrating Masson’s Trichrome, Sirius Red, and immunohistochemistry with Collagen I a. Positive areas of Masson’s Trichrome b, Sirius Red c, and Collagen I d were quantitatively analyzed. The kidney tissue lysates were subjected to immunoblot analysis with specific antibodies against Collagen I and GAPDH e. Expression level of Collagen I was quantified by densitometry and normalized with GAPDH f. Data are represented as the mean ± SEM (n = 6). Means with different superscript letters are significantly different from one another (P < 0.05). All scale bars = 20 μm.