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. 2020 Jun 17;11(6):467. doi: 10.1038/s41419-020-2673-z

Fig. 4. Inhibition of autophagy with 3-MA blocks renal EMT by regulating two transcription factors: Snail and Slug in hyperuricemic rats.

Fig. 4

The kidney tissue lysates were subjected to immunoblot analysis with specific antibodies against α-SMA, Vimentin, E-cadherin, ZO-1, and GAPDH a. Expression levels of α-SMA, Vimentin, E-cadherin, ZO-1 were quantified by densitometry and normalized with GAPDH b. Photomicrographs illustrating immunofluorescence of α-SMA and E-cadherin, respectively, costained with DAPI c. The kidney tissue lysates were subjected to immunoblot analysis with specific antibodies against Snail, Slug, and GAPDH d. Expression levels of Snail and Slug were quantified by densitometry and normalized with GAPDH e. Photomicrographs illustrating immunofluorescence co-staining of Snail and DAPI f. Data are represented as the mean ± SEM (n = 6). Means with different superscript letters are significantly different from one another (P < 0.05). All scale bars = 50 μm.