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. 2020 Jun 17;11(6):467. doi: 10.1038/s41419-020-2673-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Photomicrographs illustrating Tunel staining of the kidneys a. Quantitation of the number of Tunel-positive cells b. The kidney tissue lysates were subjected to immunoblot analysis with specific antibodies against Cleaved caspase 3, Bax, Bcl-2, and GAPDH c. Expression levels of Cleaved caspase 3, Bax, and Bcl-2 were quantified by densitometry and normalized with GAPDH d. Photomicrographs illustrating immunofluorescence staining of Cleaved caspase 3 e. Quantitation of the number of Cleaved caspase 3 positive cells f. Photomicrographs illustrating immunohistochemistry staining of Bax g. Quantitation of the number of Bax-positive cells h. Data are represented as the mean ± SEM (n = 6). Means with different superscript letters are significantly different from one another (P < 0.05). Scale bars in a, e = 50 μm and g = 20 μm.