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Cell uptake and cytotoxicity.(A,C) FCM profiles of (A) DOX and (C) NP-FITC fluorescence intensity toward K7 cells incubated with PBS, free DOX, NP-FITC/DOX, or CaNP-FITC/DOX. (B,D) The Relative GMFIs for (B) DOX and (D) NP-FITC. (E) In vitro inhibition efficacies and (F) IC50s of free DOX, NP/DOX, and CaNP/DOX against K7 cells after incubation for 48 h at pH 7.4. Data are presented as mean ± standard deviation (SD; n = 3; *P < 0.05, **P < 0.01, ***P < 0.001).
