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Pharmacokinetics and biodistribution of DOX formulations in vivo. (A) In vivo DOX pharmacokinetics after i.v. injection of free DOX, NP/DOX, or CaNP/DOX in to Sprague-Dawley rat. (B) DOX fluorescent images representing tissue distribution of DOX. (C, D) Average semiquantitative signals of major organs and tumor after i.v. injection to K7 osteosarcoma-allografted BALB/c mouse for (C) 2 or (D) 6 h. Data are presented as mean ± SD (n = 3; *P < 0.05, ***P < 0.001).
