Table 5.
The effect of APOL1 polymorphism on transmembrane properties
| Transmembrane properties | APOL1G0 | APOL1G1 | APOL1G2 |
|---|---|---|---|
| Membrane thickness | 25.2 Å | 19.8 Å | 33.0 Å |
| Transmembrane segments | Gln35-Ser64 (Tilt: 62.3°), Gly171-Ala197 (Tilt: 58.6°), Phe199-Thr221 (Tilt: 42.5°), S342-S365 (Tilt: 50.6°), Thr367-Ala394 (Tilt: 55.7°) | Ala32-Thr59 (Tilt: 64.4°), Gly171-Leu196 (Tilt: 66.5°), Glu201-Ala218 (Tilt: 58.5°), Gly231-Ser263 (Tilt: 67.5°), Pro324-Ala339 (Tilt: 40.8°), Val341-Leu359 (Tilt: 50.2°), Ala375-Tyr389 (Tilt: 44.4°) | Phe199-Glu308 (Tilt: 11.9°), Met329-Ala363 (Tilt: 62.4°) |
| Multimer hydrophobic thickness | No multimer | 30.0 Å | 24.6 Å |
| Segments | Not any. | chain A:Lys148-Leu189 (Tilt: 30.6°), chain A:Lys372-Gln393 (Tilt: 32.0°), chain C: Lys148-Leu189 (Tilt: 29.4°), chain C: Lys373-Ala394 (Tilt: 28.2°), chain B: Lys148-Leu189 (Tilt: 29.9°), chain B: Lys372-Gln393 (Tilt: 32.4°), chain E: Lys148-Leu189 (Tilt: 30.5°), chain E: Lys372-Gln393 (Tilt: 31.3°), chain D: Lys148-Leu189 (Tilt: 29.7°) chain D: Lys373-Ala394 (Tilt:27.5°) | chain A: Ser40-Met60 (Tilt: 22.7°), chain A: Gly202-Asp229 (Tilt: 54.7°), chain A: Gln237-Arg255 (Tilt: 44.0°), chain C: Glu201-Ala312 (Tilt: 45.5°), chain C: Ser314-Glu361 (Tilt: 56.1°), chain B: Asn264-Glu316 (Tilt: 35.2°), chain B: Ala339-His360 (Tilt: 51.6°) |
| overall surface area | - | 107613.7 Å2 | 111551.4 Å2 |
| buried area | 17134.0 Å2 | 12601.5 Å2 | 15862.1 Å2 |
| solvation free energy gain (ΔGint) | −12.6 kcal/mol | −48.9 kcal/mol | −54.9 kcal/mol |
| free energy of assembly dissociation (ΔGdiss) | −27.6 kcal/mol | 6.5 kcal/mol | 25.7 kcal/mol |
| rigid body entropy change at dissociation (TΔSdiss) | 60.9 kcal/mol | 60.3 kcal/mol | 61.3 kcal/mol |
| Symmetry no | Multimer not favorable | 5 | 5 |