FIG 3.

PrP^res Western blot profiles and vacuolar lesion profiles in the brains of human PrP-expressing mice (tgHu) inoculated with discrepant phenotype sCJD cases. VV2 (case 11 [▵]), MV1 (case 15 [▽] and case 16 [□]) and MV2 (case 19 [○] and case 20 [◊]) sCJD isolates were inoculated into transgenic mice that express Met₁₂₉ (tgMet), Val₁₂₉ (tgVal) human PrP and their crossbreed (tgMet/Val) (intracerebral route, 6 mice, 20 μl per mouse). After two iterative passages in tgMet, tgVal, and tgMet/Val mice, the PrP^res WB profile was established in the mouse brains by SDS-PAGE and WB with the anti-PrP monoclonal antibody Sha31 (epitope YEDRYYRE). A PrP^res type 1 isoform (MM1 sCJD isolate [case 1]) and type 2 isoform (VV2 sCJD isolate [case 2]) were included as controls on each gel. In parallel, standardized vacuolar lesion profiles in the brains of the mice that were inoculated with each isolate were established. Lesion profiles corresponding to M1^CJD (○) and V2^CJD (•) strains are presented for comparison.