. 2020 Jun 16;11(3):e00393-20. doi: 10.1128/mBio.00393-20

TABLE 3.

Transmission of sporadic CJD isolates displaying complex PrP^res profile in mice expressing the human PrP either the methionine or the valine at codon 129^a

Isolate				tgMet						tgMet/Val						tgVal
Isolate				Passage 1			Passage 2			Passage 1			Passage 2			Passage 1			Passage 2
Case	Codon 129	PrP^res type	Brain area^b	D/I^c	IncubP^d	PrP^res type	D/I	IncubP	PrP^res type	D/I	IncubP	PrP^res type	D/I	IncubP	PrP^res type	D/I	IncubP	PrP^res type	D/I	IncubP	PrP^res type
21	VV	1	Caudate	6/6	346 ± 75	1	6/6	215 ± 12	1	6/6	369 ± 52	1	6/6	245 ± 8	1	6/6	196 ± 7	2	6/6	174 ± 7	2
		2	Cerebellum	5/5	569 ± 89	1	6/6	504 ± 74	1	6/6	483 ± 23	1	6/6	504 ± 54	1	6/6	184 ± 11	2	6/6	170 ± 10	2
		2	Temp. Cort	6/6	333 ± 61	1	6/6	221 ± 8	1	6/6	385 ± 61	1	6/6	241 ± 10	1	6/6	208 ± 8	2	6/6	170 ± 9	2

22	MV	2	Caudate	6/6	518 ± 84	1	6/6	571 ± 14	1	6/6	466 ± 90	1	5/5	476 ± 26	1	6/6	180 ± 8	2	6/6	161 ± 8	2
		1+2	Cerebellum	6/6	357 ± 38	1	6/6	220 ± 5	1	6/6	396 ± 66	1	6/6	261 ± 11	1	6/6	180 ± 4	2	6/6	175 ± 5	2
		2	Occip. Cort	6/6	277 ± 50	1	6/6	210 ± 5	1	6/6	387 ± 17	1	6/6	245 ± 21	1	6/6	217 ± 23	2	6/6	164 ± 7	2

23	MV	1+2	Caudate	5/6	519 ± 88	1	6/6	212 ± 8	1	6/6	523 ± 82	1	6/6	250 ± 2	1	6/6	251 ± 66	2	6/6	168 ± 3	2
		2	Cerebellum	0/5	>750*	1	6/6	579 ± 20	1	0/6	>750*	1	6/6	484 ± 40	1	6/6	316 ± 52	2	6/6	166 ± 4	2
		1	Temp. Cort	1/6	689, 718*	1	6/6	254 ± 30	1	0/6	>750	−^e		ND		6/6	362 ± 58	1	6/6	193 ± 13	2

Three sCJD cases displaying different PRNP genotypes at codon 129 (VV, homozygous Val₁₂₉; MV, heterozygous Met/Val₁₂₉) and showing mixed type 1/type 2 PrP^res Western blot profiles in either the same or different brain areas were selected. Three brain areas from each case were inoculated (two iterative passages) in transgenic mice that express the Met₁₂₉ (tgMet) or Val₁₂₉ (tgVal) human PrP and their crossbreed (tgMet/Val) were inoculated intracerebrally (20 μl per mouse). Brains from first passage mice (PrP^res positive in the brain) were pooled and used for the second passage. The PrP^res WB isoforms (type 1 or type 2) identified in mouse brains are reported.

Abbreviations: Caudate, caudate nucleus; Temp. Cort, temporal cortex; Occip. Cort, occipital cortex.

D/I, number of diseased mice/number of inoculated mice. *, PrP^res positive in a found dead animal without TSE clinical signs.

IncubP, incubation period (time to death [in days]) shown as means ± standard deviations. An asterisk (e.g., >750*) indicates that all mice were PrP^res positive, but showed no clinical signs when killed (end of their life span). ND, not done.

−, PrP^res negative.