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. 2020 Jun 16;11(3):e00589-20. doi: 10.1128/mBio.00589-20

FIG 2.

FIG 2

LMP1 expression increases mRNA and protein levels of EV biogenesis components. mRNA was collected from normal HEK293 cells or HEK293 cells stably expressing an inducible LMP1 construct and subjected to RT-qPCR. (A and B) Relative mRNA levels of the different EV biogenesis components (CD9, CD63, CD81, Hrs, Alix, Syntenin-1, TSG101, CHMP1A, CHMP2A, CHMP2B, CHMP3, CHMP4B, CHMP4C, CHMP5, and CHMP6) were determined by normalization to GAPDH levels (for HEK293 cells) and normal HEK293 cells (for HEK293 cells stably expressing LMP1). (C) Relative mRNA levels of different EV biogenesis genes (Hrs, Alix, Syntenin-1, and TSG101) collected from EVs of HEK293 cells or HEK293 cells expressing LMP1. (D and E) Relative mRNA levels of EV biogenesis components collected from the nasopharyngeal carcinoma cell line HK1 or HK1 expressing inducible LMP1. (F) Relative mRNA levels of different EV biogenesis genes collected from EVs of HK1 cells or HK1 cells expressing LMP1. (G to J) Immunoblot analysis of cell and vesicle lysates from wild-type HEK293 or HK1 cells and HEK293 or HK1 cells expressing inducible LMP1 showing expression levels of the different EV biogenesis components from 3 independent experiments (*, P < 0.05; **, P < 0.001).