FIG 6.

Syntenin-1 and Hrs knockdowns exhibit altered LMP1 endolysosomal trafficking. (A and C) Cells expressing shRNAs were either transfected with GFP-LMP1 and then stained with Lysotracker at 24 h posttransfection or cotransfected with GFP-LMP1 and Rab7. Live-cell confocal images were acquired at 24 h posttransfection on a Zeiss microscope. (B and D) Colocalization was quantified using Pearson’s correlation coefficient (n = 8 cells). Representative maximum-projection images are shown (*, adjusted P < 0.005; **, adjusted P < 0.0001; ***, adjusted P < 0.05). Bars, 10 μm.