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. 2020 Jun 16;11(3):e00589-20. doi: 10.1128/mBio.00589-20

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Copyright © 2020 Nkosi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 7 — Knockdown of Syntenin-1 and Hrs in the nasopharyngeal carcinoma cell line HK1 impairs LMP1 vesicle secretion and packaging. HK1 cells expressing inducible shRNAs (scramble, Hrs, and Syntenin-1) and inducible GFP-LMP1 were generated. (A and B) Whole-cell (A) and EV (B) lysates were separated by SDS-PAGE and analyzed by immunoblot analysis for LMP1 and common EV markers (Alix, Hrs, calnexin, HSC70, TSG101, Syntenin-1, Flotillin-2, and CD63). (C) Semiquantitative Western blot analysis of results from more than 3 independent experiments. The data are presented relative to the value for wild-type LMP1 packaged into EVs. (D and E) Nanoparticle tracking analysis for size (D) and quantity (E). (*, adjusted P < 0.005; **, adjusted P < 0.0001).