TABLE 2.
Primers used in this study
| Primer type and name | Primer sequence | Primer descriptiona |
|---|---|---|
| Deletion constructs | ||
| AA4950 | AGGATTTGGGCAAATCGAGA | SRE1 ko primer 1 |
| AA4951 | GTCATAGCTGTTTCCTGGGGAAAGAATCGTCTCATCA | SRE1 ko primer 2 |
| AA4952 | ACTGGCCGTCGTTTTACAGGCGATGCTATCTATGGGT | SRE1 ko primer 3 |
| AA4953 | GGAACCAATAAAGCGACCCA | SRE1 ko primer 4 |
| M13F | GTAAAACGACGGCCAGT | NEO cassette flank (F) |
| M13R | CAGGAAACAGCTATGAC | NEO cassette flank (R) |
| AA3935 | CCTGAATGAACTGCAGGA | NEO internal cassette (R) |
| AA3934 | TCGATGCGATGTTTCGCT | NEO internal cassette (F) |
| Reconstitution constructs | ||
| AA5546 | CGTCGCACTAGTGAGAGGGAGAAAGCTGGC | SRE1 complement (F) |
| AA5547 | CGTCGCACTTTTGGTGGACGGGCATTAATA | SRE1 complement (R) |
| Southern probes | ||
| AA4975 | GGAACTGGCCAAATACGCAG | SRE1 Southern probe (F) |
| AA4976 | TTCCATGGTCCCTATCCATT | SRE1 Southern probe (R) |
| Fluorescent constructs | ||
| AA5514 | GTACGGATCCACTAGTATGGCCTCATTACAGGACAAGATGC | HIS-GFP-SRE1 (F) 1 |
| AA5517 | GGCGGCCGTTACTAGTACATCACGTACGTACATACAGC | HIS-GFP-SRE1 (R) 2 |
a
Abbreviations: ko, knockout; F, forward; R, reverse.