FIG 2.

Doxycycline-mediated DINO expression mimics induction by DNA damage. DINO expression as analyzed by qRT-PCR in control vector-transduced SiHa cells (basal) or treated with 0.2 μg/ml doxorubicin for 24 h (+Doxorubicin) compared to acute DINO expression by treating inducible DINO vector-transduced SiHa cells with 1 μg/ml doxycycline for 48 h (+Doxycycline) (A). Quantification of the increases in the cytoplasmic and nuclear DINO levels by qRT-PCR (B). Assessment of the relative increases in the nuclear and cytoplasmic DINO pools by qRT-PCR (C). Expression data are presented in arbitrary units (AU) and are normalized to expression of the RPLP0 housekeeping gene. Bar graphs represent means ± SEM (n = 3). ***, P < 0.001 (Student’s t test).