FIG 7.

DINO induction by DNA damage can occur independently of ATM/CHK2/TP53 DNA damage signaling. (A) The HPV16-positive SiHa (left) and CaSki (right) cervical cancer lines were treated with 0.2 μg/ml doxorubicin and either 10 μM ATM inhibitor KU-55933 (ATMi) or 5 μM CHK2 inhibitor BML-277 (CHK2i). After 24 h, expression of DINO and the canonical TP53 target gene CDKN1A was determined by qRT-PCR. (B) Doxorubicin-mediated induction of DINO and CDKN1A mRNA was also assessed in the HPV-negative C33A cell line that expresses the DNA binding-defective, TP53 R273H cancer hot spot mutant. Expression data are presented in arbitrary units (AU) and are normalized to expression of the RPLP0 housekeeping gene. Bar graphs represent means ± SEM (n = 3). ***, P < 0.001 (Student’s t test).