Fig. 2.

DIM can induce ESCC rearrangement of cytoskeleton through RhoA/ROCK1 pathway. a. ESCC cell morphology had changed from irregular long fusiform to almost round or ellipse after DIM treatment. b. WB showed DIM could downregulate the cytoskeleton-associated RhoA/ROCK1/Cofilin pathway to decrease expression of F-actin. c. Phalloidin staining of F-actin visualized the relative expression levels after various concentrations of DIM treatment. * P < 0.05, ** P < 0.01, *** P < 0.001, ns, no significance