Fig. 6.

Targeting COX2/PGE₂ pathway affects EMT process of ESCC. a. Knockdown of COX2 with specific siRNAs could reverse reverse EMT process with downregualtion of β-Catenin, Vimentin and Slug as well as MMPs and upregulation of Claudin-1. b. COX2 selective inhibitor Celecoxib synergically with DIM inhibited EMT. c. DIM inhibited transcription of COX2 measured by qPCR. d. DIM inhibited production of PGE₂ in a dose-dependent manner measured by ELISA assay. e. WB results showed that DIM could partly reverse the EMT process which could be enhanced by PGE2 treatment. * P < 0.05, ** P < 0.01, *** P < 0.001, ns, no significance