Table 1.
Comparison of the three ELISA kits used for HDV detection
| GB HDV Ab | DiaPro HDV Ab | DiaSorin ETI-AB-DELTAK-2 | |
|---|---|---|---|
| Manufacturer | General Biological Corporation | DiaPro | DiaSorin |
| Certification | TFDA MHW Medical Device Manufacturing No. 005807 | CE 0318 | CE 0459 |
| Sample (volume) | serum and EDTA-plasma(100 μl) |
serum and EDTA-plasma (100 μl) |
serum only (50 μl) |
| Principle | direct sandwich assay | two-step competitive assay | simultaneous competitive assay |
| Adsorbed Antigen | recombinant HDV antigen | recombinant HDV antigen | recombinant HDV antigen |
| HRP Conjugate | HDV small antigen | polyclonal Ab against HDV | human anti-HD Fab |
| Incubation Time |
105 min (60, 30, 15 min) |
140 min (60, 60, 20 min) |
210 min (180, 30 min) |
| Incubation Temperature | 37 °C | 37 °C or room temperature | 37 °C orroom temperature |
| Reader Wavelength |
450 nm (reading) 650 nm (blanking, if possible) |
450 nm (reading) 620–630 nm (blanking, if possible) |
450 nm (reading) 630 nm (blanking) |
| Control Well(s) | 6 (Blank*1, NC*3, PC*2) | 7 (Blank*1, NC*3, CAL*2, PC*1) | 6 (Blank*1, NC*3, PC*2) |