Fig. 6.

Confirmation of miR-320d/FoxM1 3´-UTR binding sites by dual luciferase activity reporter assay. a Two possible binding sites between miR-320d and FoxM1 3´-UTR were obtained by screening through TargetScan website. b Measurements of two luciferase activities in OE-19 cells after miR-320 mimics and 3´-UTR of FoxM1 plasmid co-transfections. NC was set as negative plasmid for miR-320 mimics. WT was the wild type of 3´-UTR of FoxM1. Mut1 was mutation of binding site 1 of 3´-UTR of FoxM1, while Mut2 was mutation of binding site 2. Mut1&2 was mutation of both two binding sites of 3´-UTR of FoxM1. The * represents significant difference from miR-320d mimics-transfected cells to EV-transfected cells (negative control) (*: P < 0.05; **: P < 0.01; ***: P < 0.001). NS represents no significance. Data are shown as mean ± SD