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. 2020 Jun 16;11:236. doi: 10.1186/s13287-020-01758-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characterization and differentiation of hDPSCs from human dental pulp tissue. a The dental pulp was extracted from the impacted third molars with forceps. b Cultured hDPSCs observed with a phase-contrast microscope. Bar = 100 μm. Representative flow cytometric histograms. Cell surface double staining was conducted with CD29, CD73, CD90, and CD105 antibodies and the CD45 antibody. CD90⁺ and CD45⁻ cells were selected, and CD73- and CD105-positive cell counts were determined