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. 2020 Jun 16;18:92. doi: 10.1186/s12951-020-00649-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — In vitro targeting and transmembrane mechanism of DPLGA@[RAW-4T1] NPs. a Flow cytometry detection results and b mean fluorescence intensity obtained in four different cell types (RAW264.7, G422, RM-1, 4T1) upon 4 h treatment with DiL-labeled PLGA@[RAW-4T1] NPs. c Confocal microscopic images of RAW264.7 cells, G422 cells, RM-1 cells, and 4T1 cells cultured with DiL dyed PLGA@[RAW-4T1] NPs. Scale bar = 50 μm. d Mean fluorescence intensity analysis in flow cytometry detection of 4T1 cells incubated with blank solution, free Dox, DPLGA NPs, DPLGA@4T1 NPs, DPLGA@RAW NPs, and DPLGA@[RAW-4T1] NPs. e Confocal microscopic observation in 4T1 cells after treatment with blank solution, Dox, DPLGA NPs, DPLGA@4T1 NPs, DPLGA@RAW NPs, or DPLGA@[RAW-4T1] NPs. f Cellular internalization of DPLGA@[RAW-4T1] NPs was scanned using a confocal microscope. Scale bar = 50 μm. g Impact of inhibitors of endocytic pathways on cell uptake of DPLGA@[RAW-4T1] NPs on 4T1 cells. Data are expressed as the mean ± SD (n = 3). * p < 0.05 was regarded to indicate a significant difference between these two groups