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. 2020 Jun 16;20:249. doi: 10.1186/s12935-020-01322-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Identification of ZFAS1 overexpression and clinical correlations in PAAD by experimental analysis. a ZFAS1 expression in PAAD (n = 83) and normal tissue samples (n = 69) determined by ISH; scale bars, 50 μm. b Different ZFAS1 expression scores (intensity*area) in PAAD (n = 83) and normal tissue samples (n = 69) measured by ISH, p < 0.05. c ZFAS1 subcellular localization and expression level. d Kaplan–Meier survival analysis of PAAD patients stratified according to their ZFAS1 expression level, p < 0.05. e ROC curve showing the AUC for ZFAS1 (0.747, 95% CI 0.670–0.814), p < 0.05. f Relative ZFAS1 expression levels in PAAD cell lines and a human pancreatic ductal epithelial cell line measured by qRT-PCR (n = 3), *p < 0.05