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. 2020 Jun 16;20:249. doi: 10.1186/s12935-020-01322-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — ZFAS1 functions as a sponge of miR-3924. a The flowchart of all bioinformatic analyses. b The binding site of ZFAS1/miR-3924 predicted by DIANA-LncBase 2.0. c Venn diagram of the target miRNAs predicted with starBase 3.0 and DIANA-LncBase 2.0. d HEK-293T cells transfected with ZFAS1 3′-UTR Wt, Mut, NC or PC reporter plasmids along with miR-3924 mimics. Relative luciferase activity was measured (n = 3), *p < 0.05. e–g Wound healing assays showing that miR-3924 inhibition reversed the effects of ZFAS1 silencing (n = 3), *p < 0.05; scale bars, 50 μm. h, i Transwell migration assays showing that miR-3924 inhibition reversed the effects caused by ZFAS1 silencing (n = 3), *p < 0.05; scale bars, 50 μm. j Relative ZFAS1 expression in cells transfected with sh-NC or sh-ZFAS1 was measured by qRT-PCR (n = 3), *p < 0.05. k, l Wound healing assays showing that miR-3924 overexpression inhibited the metastasis of BXPC3 and SW1990 cells (n = 3), *p < 0.05; scale bars, 50 μm. m Transwell migration assays showing that miR-3924 overexpression inhibited the migration of BXPC3 and SW1990 cells (n = 3), *p < 0.05; scale bars, 50 μm

Fig. 4 — ZFAS1 functions as a sponge of miR-3924. a The flowchart of all bioinformatic analyses. b The binding site of ZFAS1/miR-3924 predicted by DIANA-LncBase 2.0. c Venn diagram of the target miRNAs predicted with starBase 3.0 and DIANA-LncBase 2.0. d HEK-293T cells transfected with ZFAS1 3′-UTR Wt, Mut, NC or PC reporter plasmids along with miR-3924 mimics. Relative luciferase activity was measured (n = 3), *p < 0.05. e–g Wound healing assays showing that miR-3924 inhibition reversed the effects of ZFAS1 silencing (n = 3), *p < 0.05; scale bars, 50 μm. h, i Transwell migration assays showing that miR-3924 inhibition reversed the effects caused by ZFAS1 silencing (n = 3), *p < 0.05; scale bars, 50 μm. j Relative ZFAS1 expression in cells transfected with sh-NC or sh-ZFAS1 was measured by qRT-PCR (n = 3), *p < 0.05. k, l Wound healing assays showing that miR-3924 overexpression inhibited the metastasis of BXPC3 and SW1990 cells (n = 3), *p < 0.05; scale bars, 50 μm. m Transwell migration assays showing that miR-3924 overexpression inhibited the migration of BXPC3 and SW1990 cells (n = 3), *p < 0.05; scale bars, 50 μm