Fig. 5.

ROCK2 functions as the target of ZFAS1/miR-3924. a The differentially expressed genes in the high ZFAS1 expression group were focused primarily in the focal adhesion pathway. b The Venn diagram shows the target mRNAs predicted with GSEA, TargetScan 7.2 and miRDB. c The binding site of miR-3924/ROCK2 was predicted with TargetScan 7.2. d 293T cells were transfected with ROCK2 3′-UTR Wt, Mut, NC or PC reporter plasmids along with miR-3924 mimics, and relative luciferase activity was measured (n = 3), *p < 0.05. e–h Western blot assays showed that the expression levels of FAK, RHOA and ROCK2 in the sh-ZFAS1 and miR-3924 mimic groups were lower than those in the mock group (n = 3), *p < 0.05