Figure 7. Ezh2 mutation induces reduction in clonality.

A. Mutation frequency of V segments (n=3 mice), measured in proportion of mismatched nucleotide sites between each V segment of each sequence and its predicted germline V segment; p values Wilcoxon rank sum test.

B. Diversity curves of YFP;Cγ1-cre (WT) and YFP;Ezh2^Y641F mice generated through 1000 uniformly sampled bootstrap replicates.

C. Lineage trees of three largest WT and Ezh2^Y641F YFP mice.

D. Distribution of 1000 bootstrap replicates showing total trunk/canopy (non-trunk) branch length ratio and internal/external branch length ratio of lineage trees obtained from WT and Ezh2^Y641F YFP mice. Marginal histograms of each statistic are shown on the top and right sides.

E. IF confocal microscopy images of R26R-Confetti splenic GCs at day 10 post-SRBC immunization.

F. Heatmap representing quantification of clonal abundance from GCs derived from 4 R26R-Confetti;Cγ1-cre and 4 R26R-Confetti;Ezh2^Y641F mice. Scale from white to red denotes the fractional abundance of each clone.

G-H. Shannon entropy was calculated per GC (represented by dots, with each color representing GC from a different mouse, n WT=34, Ezh2^Y641F=39) (G) and for all GCs per mouse (H).

I. Diversity curves of R26R-Confetti mice generated as in B.

J. Distribution of lineage trees obtained from R26R-Confetti mice analyzed as in D.

See also Figure S7.