TABLE 1.
Primer sequences (5′–3′) used in quantitative polymerase chain reaction (qPCR)
| Gene | Sequence | Amplicon (bp) |
|---|---|---|
| Ngf | F‐ CCCAATAAAGGCTTTGCCAAGGAC | 78 |
| R‐ GAACAACATGGACATTACGCTATGC | ||
| Nt‐3 | F‐ GACACAGAACTACTACGGCAACAG | 184 |
| R‐ ACTCTCCTCGGTGACTCTTATGC | ||
| Bdnf | F‐ CGATTAGGTGGCTTCATAGGAGAC | 182 |
| R‐ CAGAACAGAACAGAACAGAACAGG | ||
| Gdnf | F‐ GCTGACCAGTGACTCCAATATGC | 192 |
| R‐ CCTCTGCGACCTTTCCCTCTG | ||
| Vegf | F‐ ACTTGAGTTGGGAGGAGGATGTC | 183 |
| R‐ GGATGGGTTTGTCGTGTTTCTGG | ||
| Nestin | F‐ CAAGGTCTGGTCTGGTGTATGC | 106 |
| R‐ GCTTTATTCAGGGAGGAAGAGAGG | ||
| Sox10 | F‐ ACGCAGAAAGTTAGCCGACCAG | 92 |
| R‐ CACTCTCGTTCAGCAACCTCCAG | ||
| Dcx | F‐ CGCCGCAGCAAGTCTCCAG | 185 |
| R‐ TCGCCAAGTGAATCAGAGTCATCC | ||
| β‐III tubulin | F‐ GCTGGAACGCATCAGTGTCTAC | 162 |
| R‐ GCACCACTCTGACCGAAGATAAAG | ||
| Gfap | F‐ GGGACAATCTCACACAGGACCTC | 162 |
| R‐ CCTCCAGCGACTCAACCTTCC | ||
| Actin, Beta | F‐TCTATCCTGGCCTCACTGTC | 122 |
| R‐AACGCAGCTCAGTAACAGTCC | ||
| Tnf‐α | F‐ CCACCACGCTCTTCTGTCTACTG | 149 |
| R‐ GGCTACGGGCTTGTCACTCG | ||
| Il‐1β | F‐ TTCAAATCTCACAGCAGCATCTCG | 198 |
| R‐ ACACTAGCAGGTCGTCATCATCC | ||
| Il‐6 | F‐ AGCCAGAGTCATTCAGAGCAATAC | 161 |
| R‐ GTTGGATGGTCTTGGTCCTTAGC | ||
| Il‐10 | F‐ CACCCACTTCCCAGTCAGC | 148 |
| R‐ ACCCAAGTAACCCTTAAAGTCCTG | ||
| Hprt | F‐ CCAGCGTCGTGATTAGTGATGATG | 135 |
| R‐ GAGCAAGTCTTTCAGTCCTGTCC |