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. 2020 Jun 10;7:245. doi: 10.3389/fvets.2020.00245

Figure 5.

Figure 5

Relative gene expression in duodenal tissue of 35 d broiler chickens fed different levels of calcium with or without 1-alpha-hydroxycholecalciferol supplementation (D3 + 1α; D3, respectively). (A) Calbindin d28k (CALB) (B) Mucin 2 (MUC2) (C) Vitamin D receptor (VDR) (D) Sodium-phosphate cotransporter type II b (NPTIIb). Duodenal tissue was analyzed using qPCR normalized against glyceraldehyde phosphate dehydrogenase (GAPDH; housekeeping gene) expression (n = 5). [General linear models (GLM). *p < 0.05].