| Oligonucleotides |
| crRNAs |
This study |
Table S4 |
| Recombinant DNA |
| Plasmids |
This study |
Table S5 |
| Antibodies |
| mouse monoclonal GFP antibody |
Biolegend |
Cat# 668206 |
| anti-his tag anti-body |
Thermo Fisher Scientific |
Cat# TA150087 |
| Bacterial and Virus Strains |
|
E. coli c-3000 |
ATCC |
Cat# 15597 |
| NEB 5-alpha Competent E. coli
|
New England BioLabs |
Cat# C2987H |
| BL21 Competent E. coli
|
New England BioLabs |
Cat# C2530H |
|
E. coli bacteriophage MS2 |
ATCC |
Cat# 15597-B1 |
|
Leptotrichia buccalis DSM 1135 |
ATCC |
Cat# 14201 |
|
E. coli c3000-derived strains |
This study |
Table S6 |
| Chemicals, Peptides, and Recombinant Proteins |
| LB Broth Base |
Thermo Fisher Scientific |
Cat# 12780029 |
| LB Agar |
Fisher Scientific |
Cat# BP1425-500 |
| SOC medium |
New England BioLabs |
Cat# B9020S |
| ampicillin |
Fisher Scientific |
Cat#11593027 |
| chloramphenicol |
Sigma |
Cat# C0378 |
| kanamycin |
Fisher Scientific |
Cat# 25389-94-0 |
| gentamicin |
ACROS |
Cat# 1405-41-0 |
| IPTG |
Invitrogen |
Cat# 15529019 |
| penicillin-streptomycin |
Thermo Fisher Scientific |
Cat# 10378016 |
| fetal bovine serum |
VWR Seradigm |
Cat# 97068-085 |
| LipofectAmine 3000 |
Thermo Fisher Scientific |
Cat# L3000015 |
| Opti-MEM I Reduced Serum Medium |
Thermo Fisher Scientific |
Cat# 11058021 |
| RMPI 1640 Medium |
Thermo Fisher Scientific |
Cat# 11875119 |
| protease inhibitor cockt |
Thermo Fisher Scientific |
Cat# 78425 |
| Critical Commercial Assays |
| QIAGEN Plasmid Mini Kit |
QIAGEN |
Cat# 12125 |
| myTXTL Sigma 70 Master Mix Kit |
Arbor Bioscience |
Cat# 507024 |
| AMPure XP beads |
Beckman Coulter |
Cat# A63882 |
| Dynabeads™ Sheep anti-Mouse IgG |
Invitrogen |
Cat# 11201D |
| Pierce™ His Protein Interaction Pull-Down Kit |
Thermo Fisher Scientific |
Cat# 21277 |
| Pierce™ Gaussia-Firefly Luciferase Dual Assay Kit |
Thermo Fisher Scientific |
Cat# 16181 |
| High Capacity cDNA Reverse Transcription Kit |
Thermo Fisher Scientific |
Cat# 4368814 |
| Maxima SYBR Green qPCR Master Mix |
Thermo Fisher Scientific |
Cat# K0252 |
| Direct-zol™ RNA MiniPrep kit |
Zymo Reseach |
Cat# R2052 |
| the CellTiter 96 AQueous One Solution Cell Proliferation assay |
Promega |
Cat# G3580 |
| Pierce LDH Cytotoxicity Assay Kit |
Thermo Fisher Scientific |
Cat# C20300
|
| Experimental Models: Cell Lines |
| HEK293T |
ATCC |
Cat# CRL-3216 |
