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. Author manuscript; available in PMC: 2020 Jun 17.
Published in final edited form as: FASEB J. 2020 Mar 16;34(5):6335–6350. doi: 10.1096/fj.201901951RR

Figure 1. Depletion of cGMP suppressed upregulation of RyR2 in Cnga3−/−/Nrl−/− retinas.

Figure 1.

Expression of phospho-RyR2 and Ryr2 mRNA levels in retinas of Nrl−/−, Cnga3−/−/Nrl−/−, and Cnga3−/−/Nrl−/−/Gucy2e−/− mice at P15, P30, and P60 were analyzed by immunoblotting and qRT-PCR. (A) Increased phospho-RyR2 level in Cnga3−/−/Nrl−/− retinas and deletion of retGC1 suppressed the upregulation. Shown are representative immunoblotting images of phospho-RyR2 and corresponding quantitative analysis. (B) qRT-PCR results showing increased mRNA level of RyR2 in Cnga3−/−/Nrl−/− retinas. Deletion of retGC1 suppressed the upregulation. Data are represented as mean ± SEM 3-4 assays using retinas prepared from 8-10 mice in each group (*P < 0.05, **P < 0.01).