FIG. 10.

Relative effects of single-, dual- and three-mitigator-drug regimens on levels of 33 stress response and inflammatory cytokine proteins over 7 days after TBI. Panels A–D: Protein levels for plasma, bone marrow, intestine and lung are shown, respectively. C57BL/6NTac mice received 9.25 Gy TBI. Subgroups were injected at 24 h postirradiation with JP4–039 (20 mg/kg), baicalein (50 mg/kg) or both JP4–039 (20 mg/kg) and baicalein (50 mg/kg), at 48 h with necrostatin-1 (1.65 Gy), or JP4–039 (20 mg/kg) plus baicalein (50 mg/kg) at 24 h plus necrostatin-1 (1.65 mg/kg) at 72 h. Mice were sacrificed on days 0, 1, 2, 3, 4 or 7 (n = 5 mice/time point). From each mouse, plasma, bone marrow, intestine and lungs (panels A–D) were isolated and Luminex assay performed for inflammatory proteins. (n = 5 per time point). Ir = irradiation alone; J = JP4–039 at h; B = baicalein at 24 h; JB and JP4–039 baicalein at 24 h; N = necrostatin 1 at 48 h; JN = JP4–039 at 24 h and necrostatin-1 at 72 h; BN = baicalein at 24 h and necrostatin-1 at 72 h; JBN JP4–039 and baicalein at 24 h and necrostatin-1 at 72 h. Significant increases in gene expression compared to day 0 are indicated in red; significant decreases in gene expression compared to day 0 are indicated in green. Data for protein expression in plasma, lung, intestine and bone marrow are summarized with the mean and standard deviation for each treatment at each time point. The two-sided two-sample t test was used to compare all days of each experimental group (including the control) to day 0 (nonirradiated baseline) of the control group.