FIG. 3.

Signature-directed optimal time of baicalein administration to C57BL/6 mice at 24 h after TBI. To determine when radiation-induced ferroptosis begins as an indicator of when to administer baicalein, C57BL/6NTac mice received 9.25 Gy TBI. At 24 h postirradiation, mouse subgroups were I.M. injected with JP4–039 (20 mg/kg), baicalein (50 mg/kg) or both JP4–039 and baicalein. On days 0, 1 and 2, mice were sacrificed and bone marrow, plasma and intestine isolated. Panel A: Western blot analysis was performed for the expression of Acyl-CoA-synthase (ALSC) and glutathione peroxidase (GPX4) in intestine, lung and bone marrow. Red arrows indicate the onset of ferroptosis, as demonstrated by increased ALSC and decreased GPX4. Panel B: Densitometric normalization of ALSC and GPX4 gene expression was by comparison to GADPH expression. *Onset of ferroptosis. P < 0.05 compared to 9.25 Gy TBI.