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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Radiat Res. 2020 Mar 5;193(5):435–450. doi: 10.1667/RR15486.1

FIG. 4.

FIG. 4.

Administration of baicalein at 24 h along with JP4–039 delays signature-directed RIP3 phosphorylation to 72 h after TBI in intestine, lung and bone marrow. C57BL/6NTac mice received 9.25 Gy TBI, then injected 24 h later with JP4–039 (20 mg/kg), baicalein (50 mg/kg) or both JP4–039 (20 mg/kg) and baicalein (50 mg/kg). Necrostatin (1.65 mg/kg) was injected at 48 h if injected alone or 72 h if mice were previously administered JP4–039 or baicalein. Mice were sacrificed on days 0, 1, 2, 3 or 4 followed by isolation of bone marrow, intestine and plasma. Panel A: Western Blot analysis for increased p-RIP3 as an indicator of necroptosis (red arrow) was performed. Panel B: Densitometric normalization of p-RIP3 expression by comparison with GADPH was performed. *Onset of necroptosis.