Figure 2.

Adenovirus-mediated overexpression of proximal tubule-specific, mitochondria-targeting mito-ECFP/Ang II increases the expression of major mitochondrial electron transport chain proteins in mPCT cells. A & D, Western blot image showing the quality and abundance of mitochondrial complex proteins in freshly isolated mitochondria vs. cell lysates. B & E, Western blot image showing increased all of five mitochondrial complex proteins in mPCT cells expressing mito-ECFP/Ang II and the effects were attenuated by losartan, the AT₁ receptor blocker. C & F, Western blot image showing that the mito-ECFP/Ang II-induced expression of mitochondrial complex proteins was attenuated by concurrent expression of mito-AT₂R/GFP in mPCT cells. *P<0.05 or **P<0.01 vs. cell lysate or control cells; ⁺P<0.05 or ⁺⁺P<0.01 vs. mito-ECFP/Ang II. Data represent results from three separate experiments.