Skip to main content
. Author manuscript; available in PMC: 2020 Jun 17.
Published in final edited form as: J Immunol. 2019 Jun 19;203(2):379–388. doi: 10.4049/jimmunol.1900276

Figure 1. Ab production by mice deficient in C3ar1/C5ar1 is impaired.

Figure 1.

WT, C3ar1−/−C5ar1−/−, and Daf1−/− mice were immunized subcutaneously with ova/IFA (n=5). A) Flow diagram of experimental design. Blood was drawn at five time points: 1 d pre-immunization and 8, 13, 21, and 30 d post-immunization with ova. Mice were boosted with ova/IFA 1 wk prior to 30 d bleed. (AU = relative absorbance units). B) Plasma from WT and C3ar1−/−C5ar1−/− mice 21 d post-immunization assayed for ova-specific total Ig. C) 21 d plasmas from WT and C3ar1−/−C5ar1−/− mice assayed for ova-specific anti-IgM, -IgG1, -IgG2a, -IgG2b, IgG2c, IgG3, -IgA, -Ig-κ, and -Ig-λ Abs. Inset: ratios of IgM and IgG isotypes in the three genotypes. D) Plasmas from WT and Daf1−/−7 d post-immunization assayed for ova-specific Ig. E) 7 d WT and Daf1−/− plasmas assayed for anti-ova-Ig isotypes as in C) above.