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. Author manuscript; available in PMC: 2021 Jun 8.
Published in final edited form as: Dev Cell. 2020 May 14;53(5):503–513.e5. doi: 10.1016/j.devcel.2020.04.012

Figure 2. Snai2 deletion impairs BM MSPC pool size and activity.

Figure 2.

(A) Quantification of total BMNCs from femurs of wild type (+/+) and Snai2-deficient (−/−) mice. (B-D) FACS plot of CD45− Ter119− CD31− BM cells and quantifications showing reduced CD51+PDGFR + (B) and Nes-GFP+ MSPCs (C) in the BM of Snai2− /− mice. EC, endothelial cells, defined as CD45− Ter119−CD31+. (E) Mesensphere-forming capacity of Snai2+/+ and Snai2− /− BM Nes-GFP+ MSPCs. (F) Sphere-forming units from femurs of Snai2+/+ and Snai2− /− mice. (G) Bright-field images of mesenspheres formed by Snai2+/+ and Snai2− /− BM Nes-GFP+ MSPCs. (H and I) immunofluorescence images from frozen sections and quantifications of Osteocalcin+ (Ocn+) osteoblasts (H) and BODIPY+ Perilipin+ adipocytes (I) in control and Snai2−/− femurs. Scale bars =100μm. (J) Micro-CT 3D reconstruction of femurs from control and Snai2−/− mice. (K) Micro-CT analysis of femurs from control and Snai2− /− mice (n=4 mice). BS, bone surface. BV, bone volume. TV, total (tissue) volume.