KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-mouse CD51 PE | eBioscience | Cat# 12-0512-83 |
| anti-mouse CD140a APC | eBioscience | Cat# 17-1401-81 |
| anti-mouse Ly-6A/E (Sca1) APC | eBioscience | Cat# 17-5981-83 |
| anti-mouse Ly-6A/E (Sca1) PE-Cy7 | eBioscience | Cat# 25-5981-82 |
| anti-mouse Ly-6A/E (Sca1) Alexa Fluor 700 | eBioscience | Cat# 56-5981-82 |
| anti-mouse CD117 (c-Kit) BV 421 | Biolegend | Cat#105828 |
| anti-mouse CD117 (c-Kit) PE-Cy7 | Biolegend | Cat#105814 |
| anti-mouse CD48 biotin | eBioscience | Cat#13-0481-85 |
| anti-mouse CD48 PerCP-eFluor 710 | eBioscience | Cat#46-0481-82 |
| anti-mouse CD48 FITC | eBioscience | Cat#11-0481-82 |
| anti-mouse CD41 APC | eBioscience | Cat#17-0411-82 |
| anti-mouse CD41 FITC | eBioscience | Cat#11-0411-81 |
| anti-mouse CD45.2 PE | eBioscience | Cat#12-0454-82 |
| anti-mouse CD45.2 PerCP-Cy5.5 | eBioscience | Cat#45-0454-82 |
| anti-mouse CD45.1 FITC | eBioscience | Cat#11-0453-85 |
| anti-mouse CD45.1 APC eFluor 780 | eBioscience | Cat#47-0453-82 |
| anti-mouse CD4 PE-Cy7 | eBioscience | Cat# 25-0041-82 |
| anti-mouse CD8a PE-Cy7 | eBioscience | Cat# 25-0081-82 |
| anti-mouse B220 APC eFluor 780 | eBioscience | Cat# 47-0452-82 |
| anti-mouse CD11b Alexa Fluor 647 | Biolegend | Cat# 101218 |
| anti-mouse CD31 Alexa Fluor 647 | Biolegend | Cat# 102516 |
| anti-mouse CD144 Alexa Fluor 647 | Biolegend | Cat# 138006 |
| anti-mouse Ki-67 eFluor 660 | eBioscience | Cat# 50-5698-82 |
| anti-mouse CD34 PE | Biolegend | Cat# 119308 |
| anti-mouse FcRII/III PerCP-eFluor 710 | eBioscience | Cat# 46-0161-82 |
| anti-mouse CD150 PE | Biolegend | Cat# 115904 |
| anti-mouse Ter119 PerCP-Cy5.5 | eBioscience | Cat# 45-5921-82 |
| anti-mouse Lineage panel cocktail biotin | BD Biosciences | Cat# 559971 |
| Streptavidin APC eFluor 780 | eBioscience | Cat# 47-4317-82 |
| Streptavidin eFluor 450 | eBioscience | Cat# 48-4317-82 |
| Streptavidin BV 570 | Biolegend | Cat# 405227 |
| anti-mouse osteocalcin | Takara | Cat# R21C-01A |
| anti-perilipin | Cell Signaling | Cat# D1D8 |
| Mouse Osteopontin (OPN) Affinity Purified Polyclonal Ab | R&D Systems | Cat# AF808 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| BODIPY 493/503 | Molecular Probes | Cat# D3922 |
| 5-Fluorouracil (5-FU) | Sigma-Aldrich | Cat# F6627 |
| PolyI:C | Invitrogen | Cat# tlrl-pic |
| DAPI (4’,6-diamino-2-phenylindole) | Sigma-Aldrich | D9542 |
| Hoechst 33342 | Sigma-Aldrich | B2261 |
| Collagenase IV | Gibco | Cat# 17104-019 |
| Dispase | Gibco | Cat# 17105-041 |
| DNase I | Sigma-Aldrich | Cat# DN25 |
| MesenCult stimulatory supplement | StemCell Technologies | 05502 |
| Recombinant murine EGF | PeproTech | AF-315-09 |
| Recombinant murine Oncostatin M (OSM) | R&D Systems | 495-MO-025 |
| Recombinant human IGF-1 | R&D Systems | 291-G1-200 |
| Recombinant murine PDGF-AA | PeproTech | 315-17 |
| Recombinant human FGF-basic | R&D Systems | 233-FB-025 |
| Chick Embryo Extract, Ultrafiltrate (CEE) 20ml | USBiological | C3999 |
| Human Endothelial-SFM | Gibco | 11111-044 |
| DMEM/F12, GlutaMAX | Gibco | 10565-018 |
| Critical Commercial Assays | ||
| RNA to cDNA EcoDry™ Premix system | Clontech | 639546 |
| Dynabeads® mRNA DIRECT™ Micro Kit | Invitrogen | 61012 |
| Deposited Data | ||
| RNA-seq | GSE142705 | N/A |
| Oligonucleotides used for qRT-PCR | ||
| Gapdh-F: TGTGTCCGTCGTGGATCTGA | Eurofins Genomics | N/A |
| Gapdh-R: CCTGCTTCACCACCTTCTTGA | Eurofins Genomics | N/A |
| Snai2-F: TGGTCAAGAAACATTTCAACGCC | Eurofins Genomics | N/A |
| Snai2-R: GGTGAGGATCTCTGGTTTTGGTA | Eurofins Genomics | N/A |
| Cxcl12-F: TGCATCAGTGACGGTAAACCA | Eurofins Genomics | N/A |
| Cxcl12-R: CACAGTTTGGAGTGTTGAGGAT | Eurofins Genomics | N/A |
| Kitl-F: GAATCTCCGAAGAGGCCAGAA | Eurofins Genomics | N/A |
| Kitl-R: GCTGCAACAGGGGGTAACAT | Eurofins Genomics | N/A |
| Spp1-F: TCCCTCGATGTCATCCCTGTTG | Eurofins Genomics | N/A |
| Spp1-R: GGCACTCTCCTGGCTCTCTTTG | Eurofins Genomics | N/A |
| Angpt1-F: CTCGTCAGACATTCATCATCCAG | Eurofins Genomics | N/A |
| Angpt1-R: CACCTTCTTTAGTGCAAAGGCT | Eurofins Genomics | N/A |
| Il7-F: TTCCTCCACTGATCCTTGTTCT | Eurofins Genomics | N/A |
| Il7-R: AGCAGCTTCCTTTGTATCATCAC | Eurofins Genomics | N/A |
| Vcam1-F: GACCTGTTCCAGCGAGGGTCTA | Eurofins Genomics | N/A |
| Vcam1-R: CTTCCATCCTCATAGCAATTAAGGTG | Eurofins Genomics | N/A |
| Runx2-F: TTACCTACACCCCGCCAGTC | Eurofins Genomics | N/A |
| Runx2-R: TGCTGGTCTGGAAGGGTCC | Eurofins Genomics | N/A |
| Cdkn1a-F: CGAGAACGGTGGAACTTTGAC | Eurofins Genomics | N/A |
| Cdkn1a-R: CAGGGCTCAGGTAGACCTTG | Eurofins Genomics | N/A |
| Bbc3-F: CTACCTCTGCGCCCCCAC | Eurofins Genomics | N/A |
| Bbc3-R: CGACTCTAAGTGCTGCTGGG | Eurofins Genomics | N/A |
| Trp53-F: GCGTAAACGCTTCGAGATGTT | Eurofins Genomics | N/A |
| Trp53-R: TTTTTATGGCGGGAAGTAGACTG | Eurofins Genomics | N/A |
| Foxc1-F: CACTCGGTGCGGGAAATGT | Eurofins Genomics | N/A |
| Foxc1-R: GGTACAGAGACTGACTGGCA | Eurofins Genomics | N/A |
| Experimental Models: Organisms/Strains | ||
| C57BL/6-Gt(ROSA)26Sortm1(HBEGF)Awai/J | The Jackson Laboratory | RRID:IMSR_JAX:007900 |
| B6; FVB-Tg(Cspg4-cre)1Akik/J | The Jackson Laboratory | RRID:IMSR_JAX:008538 |
| B6.129-Leprtm2(cre)Rck/J | The Jackson Laboratory | RRID:MGI: 3776164 |
| B6.Cg-Tg(Mx1-cre)1Cgn/J | The Jackson Laboratory | RRID:IMSR_JAX:003556 |
| Spp1Tm1Blh/J | The Jackson Laboratory | JAX: 004936 |
| C57BL/6 (CD45.2) | National Cancer Institute | |
| Bl6-Ly5.1 (CD45.1) | The Jackson Laboratory | JAX: 002014 |
| Software and Algorithms | ||
| SlideBook | Intelligent Imaging Innovations | RRID:SCR_014300 |
| FlowJo | Tree Star | RRID:SCR_008520 |
| FACS Diva 6.1 | BD Biosciences | RRID:SCR_001456 |
| Gene Set Enrichment Analysis | Broad Institute | RRID:SCR_003199 |
| Ingenuity Pathway Analysis | QIAGEN | RRID:SCR_008653 |
| GraphPad Prism | GraphPad Software | RRID:SCR_002798 |