Figure 2.

Intact mAb-1 was enzymatically digested using GingisKHAN in the upper hinge region to generate Fab and Fc domains. The digested domains were analyzed by CEX using pH gradient elution as described in the method section. The CEX profile of digested mAb-1contained three dominant peaks with distinct intrinsic fluorescence. (a) CEX chromatogram of digested domains of mAb-1. (b) Fluorescence profile of the three major peaks of the digested mAb-1: peak-A in red (330 nm/350 nm = 1.15), peak-B in blue (330 nm/350 nm = 1.38), peak-C in black (330 nm/350 nm = 1.58).