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. 2020 May 20;12(1):1763138. doi: 10.1080/19420862.2020.1763138

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© 2020 Bristol Myers Squibb. Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Analysis of mAb-1 using HIC. mAb-1 demonstrated two main peaks (peak I and peak II) in HIC analysis, and the two main peaks exhibited different intrinsic fluorescence. pH dependence of the interconversion of peak-I and peak-II were demonstrated using HIC by increasing the initial pH of the mobile phase. (a) HIC chromatographs of intact mAb-1 using acidic (pH 4.6) mobile phase. (b) HIC chromatographs of intact mAb-1 using near neutral mobile phase (pH 6.4). (c) Fluorescence profile of peak-I and peak-II.