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. 2020 Feb 28;1:28–41. doi: 10.1016/j.jvssci.2020.02.001

Supplementary Table.

Primer sequences for monocyte-derived macrophage (MDM) gene polymerase chain reaction (PCR) and exosome microRNA (miRNA) PCR

Gene Primer sequence/assay ID (F = Forward, R = Reverse)
TNFa F: AGAGGGCCTGTACCTCATCTACTC
R: GGTTGACCTTGGTCTGGTAGGA
MCP1 F: CAGCAGCAAGTGTCCCAAAG
R: GAATCCTGAACCCACTTCTGCTT
CXCL10 ID: Hs.58.39328322.gs (IDT Technologies)
IL10 F: GGCGCTGTCATCGATTTCTT
R: GTAGATGCCTTTCTCTTGGAGCTT
CCL17 ID: Hs.PT.58.159549 (IDT Technologies)
MRC1 ID: Hs.PT.58.15093573 (IDT Technologies)
UBC F: ATTTGGGTCGCGGTTCTTG
R: TGCCTTGACATTCTCGATGGT
HPRT F: CAAGCTTGCTGGTGAAAAGGA
R: TGAAGTACTTATAGTCAAGGGCATATC
miRNA Primer sequence/catalog No. (Qiagen)
hsa-miR-21-5p Sequence: 5’UAGCUUAUCAGACUGAUGUUGA
Cat #: YP00204230
hsa-miR-23b-3p Sequence: 5’AUCACAUUGCCAGGGAUUACC
Cat #: YP00204790
hsa-miR-122-5p Sequence: 5’UGGAGUGUGACAAUGGUGUUUG
Cat #: YP00205664
hsa-miR-126-3p Sequence: 5’UCGUACCGUGAGUAAUAAUGCG
Cat #: YP00204227
hsa-miR-143-3p Sequence: 5’UGAGAUGAAGCACUGUAGCUC
Cat #: YP00205992
hsa-miR-145-5p Sequence: 5’GUCCAGUUUUCCCAGGAAUCCCU
Cat #: YP00204483
hsa-miR-146a-5p Sequence: 5’UGAGAACUGAAUUCCAUGGGUU
Cat #: YP00204688
hsa-miR-146b-5p Sequence: 5’UGAGAACUGAAUUCCAUAGGCU
Cat #: YP00204553
hsa-miR-155-5p Sequence: 5’UUAAUGCUAAUCGUGAUAGGGGU
Cat #: YP00204308
hsa-miR-181b-5p Sequence: 5’AACAUUCAUUGCUGUCGGUGGGU
Cat #: YP00204530
hsa-miR-195-5p Sequence: 5’UAGCAGCACAGAAAUAUUGGC
Cat #: YP00205869
hsa-miR-208a-3p Sequence: 5’AUAAGACGAGCAAAAAGCUUGU
Cat #: YP00205619
hsa-miR-219-5p Sequence: 5’UGAUUGUCCAAACGCAAUUCU
Cat #: YP00204780
hsa-miR-221-3p Sequence: 5’AGCUACAUUGUCUGCUGGGUUUC
Cat #: YP00204532
hsa-miR-222-3p Sequence: 5’AGCUACAUCUGGCUACUGGGU
Cat #: YP00204551
hsa-miR-1202 Sequence: 5’GUGCCAGCUGCAGUGGGGGAG
Cat #: YP00206001