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. 2020 May 29;11:1123. doi: 10.3389/fmicb.2020.01123

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Copyright © 2020 Velazquez-Salinas, Pauszek, Holinka, Gladue, Rekant, Bishop, Stenfeldt, Verdugo-Rodriguez, Borca, Arzt and Rodriguez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro characterization of rNJ0612NME6 and M51R viruses in porcine monocyte-derived macrophages cell cultures (PM-MQC). (A) Multistep growth curves were performed in PM-MQC to compare virus yields between both viruses at specific times post-infection, using at initial MOI = 0.01. Differences in the transcriptional immune gene response induced by different viruses on PM-MQC, were assessed by qRT-PCR. RQ values represent relative quantities of mRNA accumulation (estimated by 2^−ΔΔCt) with their corresponding SD in (B) type I, II and III IFN, (C) cytokines and chemokines, (D) Interferon regulatory factors, and transcription factors, and (E) Interferon stimulated genes. All experiments were conducted in triplicates.