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. 2020 Apr 2;61(3):399–409. doi: 10.1093/jrr/rraa017

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© The Author(s) 2020. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — The different effects of X-ray and carbon ion irradiation on the cell cycle phase distribution in MDA-MB-231 and MCF-7 cells. The cells were irradiated by X-rays or carbon ions at doses of 0, 2, 4 and 8 Gy, and then the cell cycle phase distribution was determined by flow cytometry 48 h after irradiation. The DNA fluorescence histograms show the distribution of specific cell populations in the G₁, S and G₂/M phases in MDA-MB-231 (A) and MCF-7 (D) cells. The stacked plot shows the percentage of cells in the G₁, S and G₂/M phases in MDA-MB-231 (B) and MCF-7 (E) cells. The bar graphs show the percentage of MDA-MB-231 cells (C) and MCF-7 cells (F) in the G₂/M phases. Cells were stained using PI and subjected to flow cytometric analysis, which included 20 000 events. Data are the mean ± SD of three independent experiments. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 by one-way ANOVA.