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. 2020 Apr 2;61(3):399–409. doi: 10.1093/jrr/rraa017

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© The Author(s) 2020. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Effects of X-ray or carbon ion radiation on the expression level of γ-H2AX in MDA-MB-231 and MCF-7 cells. The blots show the expression levels of DNA DSB marker γ-H2AX in MDA-MB- 231 (A) and MCF-7 (B) cells measured by western blotting assay 48 h after X-ray or carbon ion radiation at doses of 0, 2, 4 and 8 Gy. The bar graphs show the relative levels of γ-H2AX in MDA-MB-231 (A) and MCF-7 (B) cells. β-Actin was used as the internal control. Data are the mean ± SD of three independent experiments. ^**P < 0.01 and ^***P < 0.001 by one-way ANOVA.