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. 2020 Apr 2;61(3):399–409. doi: 10.1093/jrr/rraa017

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© The Author(s) 2020. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Apoptosis induced by X-rays or carbon ions in MDA-MB-231 and MCF-7 cells determined by flow cytometry 48 h after irradiation. The flow cytometric dot plots show the percentage of specific cell populations (live, early apoptosis and late apoptosis) in MDA-MB-231 (A) and MCF-7 (C) cells irradiated with X-rays or carbon ions at doses of 0, 2, 4 and 8 Gy. The bar graphs show the percentage of late apoptotic MDA-MB-231 (B) and MCF-7 (D) cells. Cells were double-stained with annexin V/PI to detect cells undergoing early and late apoptosis, which collected 10 000 events. Data are the mean ± SD of three independent experiments. ^*P < 0.05, ^**P < 0.01 and ^***P < 0.001 by one-way ANOVA.